Abstract: In the current study,3 bacterial microbiota samples of Zhaguangjiao collected from Dangyang Hubei province were studied by single molecule real-time sequencing technique. Lactobacillus was the dominant bacteria genus with the relatively abundant of 94.25%. At the species level,relatively abundant species more than 1.0% of total bacteria in this population included L. pobuzihii,L. alimentarius,L. versmoldensis,L. parabrevis,L. brevis,L. koreensis,and L. paralimentarius of Lactobacillus,with the relative abundance of 32.31%,23.17%,22.01%,8.90%,3.31%,1.63% and 1.05%. And W. paramesenteroides of Weissella had 1.05% of total bacteria. At the OTU level,the result indicated that 6 OTUs shared by all samples,and the cumulative average relative was 29.58% of total qualified sequences. The results also showed that the bacterial microbiota of Zhaguangjiao samples main consisted by several bacterial species belonged to Lactobacillus,and the three samples shared a large number of core bacterial microbiome.