Abstract: In this paper, the optimum conditions of Schisandra chinensis glycoprotein Sevage method were studied.Glycoprotein of Schisandra chinensis was obtained from alkali extraction and acid precipitation method. Protein clearance rate and polysaccharide loss rate were used as the index of investigation, the effects of the ratio of chloroform and n-butanol, the proportion of sample solution and Sevage reagent, the shaking time and the number of proteins on the removal of protein removal were investigated. On the basis of single factor test, orthogonal design and response surface test were used to optimize the process conditions, the results showed that the ratio of chloroform to n-butanol was 3:1, the ratio of sample to Sevage was 3:1, shaking time of 14 minutes, deproteinization times 3 times, under these conditions, the average clearance rate of protein was 30.09%, and the average loss rate of polysaccharide was 4.71%, the average score for the comprehensive score was 88.11.In vitro antioxidant activity showed that Schisandra chinensis glycoprotein had strong ability to scavenge DPPH free radical and superoxide anion(IC₅₀ 1.08 mg/mL and 0.80 mg/mL), and had some reduction ability. The results showed that the response surface method was simple and efficient, and the Schisandra chinensis glycoprotein had its antioxidant activity in vitro and deserved further study.