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中国精品科技期刊2020
李伟程, 侯强川, 于洁, 孙志宏, 孙天松. 传统发酵乳制品中微生物多样性研究[J]. 食品工业科技, 2018, 39(1): 131-136. DOI: 10.13386/j.issn1002-0306.2018.01.025
引用本文: 李伟程, 侯强川, 于洁, 孙志宏, 孙天松. 传统发酵乳制品中微生物多样性研究[J]. 食品工业科技, 2018, 39(1): 131-136. DOI: 10.13386/j.issn1002-0306.2018.01.025
LI Wei-cheng, HOU Qiang-chuan, YU Jie, SUN Zhi-hong, SUN Tian-song. Study on the diversity of microbial in traditional fermented dairy products[J]. Science and Technology of Food Industry, 2018, 39(1): 131-136. DOI: 10.13386/j.issn1002-0306.2018.01.025
Citation: LI Wei-cheng, HOU Qiang-chuan, YU Jie, SUN Zhi-hong, SUN Tian-song. Study on the diversity of microbial in traditional fermented dairy products[J]. Science and Technology of Food Industry, 2018, 39(1): 131-136. DOI: 10.13386/j.issn1002-0306.2018.01.025

传统发酵乳制品中微生物多样性研究

Study on the diversity of microbial in traditional fermented dairy products

  • 摘要: 为了探究传统发酵乳制品中核心菌群,本研究采用454焦磷酸测序技术对传统发酵酸牛奶、酸马奶和酸驼奶样品中微生物的群落多样性和丰度进行了分析。研究发现,在三份传统发酵乳制品中存在一定共有微生物群。这部分共有微生物的OTU(可分类操作单元)数目较少,但拥有较大比例的序列数。在门水平上,三份样品细菌和真菌菌门分别以Firmicutes(硬壁菌门)和Ascomycota(子囊菌门)为主。在属水平上,酸牛奶样品中的细菌属以Lactococcus(乳球菌属)为主,真菌菌属以Galactomyces(耐碱酵母属)为主;酸马奶样品中的细菌属以Lactobacillus(乳杆菌属)为主,真菌属以Vanderwaltozyma(范氏酵母属)为主;酸驼奶样品的优势细菌属以Lactobacillus(乳杆菌属)为主,优势真菌属以Galactomyces(耐碱酵母属)为主。在三份样品共有菌群中LactococcusGalactomyces、LactococcusLeuconostoc(明串珠菌属)的相对含量存在显著负相关(p<0.05),LeuconostocGalactomyces、Gibberella(赤霉菌属)与Prevotella(普氏菌属)的相对含量存在显著正相关(p<0.05)。结果表示,在三份传统发酵乳制品中存在共有微生物群落,且该微生物群群落之间存在一定相关关系。

     

    Abstract: In order to investigate the core floras in traditional fermented dairy products, 454 pyrosequencing was used to analyze the microbial abundance and the community diversity in the traditional fermented yogurt, koumiss and kurut samples in present study. The result showed there were core floras in three traditional fermented dairy products. The number of OTU of this flora part was less, but exists a large number of sequences.Firmicutes was the dominant phylum of bacterium and Ascomycota was the dominant phylum of fungi in the three samples. At genus level, Lactococcus was the dominant genera in bacterium and Galactomyces was the dominant genera in bacterium in the yogurt sample. Lactobacillus and Vanderwaltozyma were there respectively dominating bacteria and fungi, in the koumiss sample.And Lactobacillus was the dominant genera in bacterium and Galactomyces was the dominant genera in bacterium in the kurut sample. At the same time, the relative abundance of Lactococcus and Galactomyces, Lactococcus and Leuconostoc core floras existed significantly negative correlation(p<0.05), Leuconostoc and Galactomyces, Gibberella and Prevotella existed significant positive correlation(p<0.05)in three traditional fermented dairy products common floras. The result showed that core floras were exist in three taditional fermenterd dairy products and they had correlations with each other.

     

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