Abstract: In this paper, roxithromycin ( ROX) molecularly imprinted macroporous D101 resin ( ROX-MIP-D101) was synthesized by free-radical solution polymerization process with ROX as template molecule, acrylamide as the functional monomer, ethylene glycol dimethacrylate as crosslinker, 2, 2-azobisisobutyronitrile as initiator, and D101 resin as carrier. And the structure was characterized by infrared spectroscopy ( IR) and scanning electron microscope ( SEM) . The adsorption properties of ROX-MIP-D101 on roxithromycin were studied by static binding experiment and dynamic binding test, and its selective adsorption properties was investigated by selective adsorption test with erythromycin as the competing substrate.Results showed that the equilibrium adsorption capacity of ROX-MIP-D101 on ROX was 253 mg/g which was obviously higher than that of NIP-D101 ( 211 mg/g) , showing that the adsorption capacity of ROX-MIP-D101 was better than that of NIP-D101.The separation factor of ROX-MIP-D101 on ROX and EM was 1.26, showing that ROX-MIP-D101 displayed high recognition ability to ROX.The separation factor of NIP-D101 on ROX and EM was 1.01, showing no recognition ability to ROX.ROX-MIP-D101 also exhibited a good regenerability and reusability. The average recovery of water spiked with ROX standard was90.8%.