Abstract: By the application of headspace solid phase microextraction ( HS-SPME) coupling with gas chromatography mass spectrometry ( GC-MS) , the aroma constituents, amino acid, monosaccharide and total sugar content in two kinds longan juice, which were made from Chuliang and Shixia breed were measured and analyzed in this study. Results indicated the primary aroma constituents in these two juices were terpenes, alcohols and esters, with abundant contents of γ-aminobutyric acid and sugar, however, the electronic nose technology was superior in distinguishing these two different breeds of longan.