Abstract: 12 samples of Douzhir ( D groups) and Ma tofu ( M groups) were collected from three manufacturers.High-throughput sequencing was used to analyze composition and diversity of bacterial community.Based on this method, dominant bacteria were screened and identified using mung bean juice as medium. In both Douzhir and Ma tofu, dominant bacteria belonged to Firmicutes, Proteobacteria and Bacteroidetes. In Douzhir of three manufacturers, predominant genera were Lactococcus, Streptococcus, Klebsiella and Lactobacillus.In Ma tofu of three manufacturers, predominant genera were Lactococcus, Lactobacillus and Acetobacter. After sequencing of 16 S rRNA, they belong to Lactococcus lactis subsp. Lactis and Acetobacter indonesiensis respectively.These two strains had important significance for industrial fermentation production of Beijing Douzhir.