Abstract: Bacteriocin CAMT2, produced by fermentation of Bacillus amyloliquefaciens ZJHD3-06, was regard as research subject, and Staphylococcus aureus, Listeria monocytogenes, Escherichia coli, Vibrio parahemolyticus, Bacillus subtilis were used as indicator bacteria.Anti-bacterial activity of bacteriocin CAMT2 treated by different temperatures, p H, proteases, and organic solvents were determined with oxford cup method. Besides, the effects of several hydrophobic substanceson emulsification property of CAMT2 were investigated. The results showed that CAMT2 had relatively high thermo-stability that after being treated under 100 ℃ for 20 min, and above 60% of its anti-bacterial activity was still retained.Within the range of p H6 ¹0, the antimicrobial activity still kept stability, while antimicrobial activity had certain decrease, and active loss rates ranged from9.4% to 51.9% in p H2 and 4. The sensitivities of CAMT2 to 4 different proteases were different. High tolerance to dimethylformamide and dichloromethane was shown.The result also showed the anti-bacterial activity of bacteriocin CAMT2 on indicator bacteria, and thus its certain spectrum of anti-bacterial action.Those properties of CAMT2 laid a foundation of theory for further development and application.