出芽短梗霉产酸性多糖的CTAB法制备工艺优化及其与普鲁兰多糖的性质比较

郝婧; 童群义

doi:10.13386/j.issn1002-0306.2017.11.022

出芽短梗霉产酸性多糖的CTAB法制备工艺优化及其与普鲁兰多糖的性质比较

郝婧,
童群义

江南大学食品学院

详细信息

作者简介:
郝婧 (1992-) , 女, 在读研究生, 研究方向:碳水化合物科学与工程, E-mail:wjhaojing@163.com。;

童群义 (1963-) , 男, 博士, 教授, 研究方向:碳水化合物科学与工程, E-mail:tqyjn@163.com。;

中图分类号: TS201.2
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出版历程
- 收稿日期: 2016-11-17

Optimization of process in extracting acidic polysaccharide from Aureobasidium pullulans using CTAB as precipitant and comparison of its properties to pullulan

School of Food Science and Technology, Jiangnan University

摘要

摘要: 采用十六烷基三甲基溴化铵(CTAB)法,制备出芽短梗霉酸性多糖,并比较了其与普鲁兰多糖的溶解性与抗氧化活性。经过单因素实验与正交实验确定制备出芽短梗霉产酸性多糖的最佳工艺条件为:质量浓度3%的CTAB溶液与初始多糖溶液体积比1∶1,初始多糖溶液质量浓度7%,沉淀12 h。该条件下,出芽短梗霉产生的酸性多糖得率为5.01%。该方法保持了出芽短梗霉酸性多糖与普鲁兰多糖的结构完整性,为该多糖的后续研究提供参考。酸性多糖与普鲁兰多糖性质比较实验结果表明,出芽短梗霉产生的中性普鲁兰多糖易溶于水,酸性多糖微溶于水;二者的抗氧化活性在0~1 mg/m L范围内无显著性差异,其还原能力、对超氧阴离子自由基以及羟基自由基清除作用均随溶液浓度的提高而增加,为出芽短梗霉酸性多糖的进一步开发与应用提供理论参考。
- 十六烷基三甲基溴化铵(CTAB) /
- 出芽短梗霉 /
- 酸性多糖 /
- 普鲁兰多糖 /
- 性质
Abstract: Acidic polysaccharide secreted by Aureobasidium pullulans was separated using cetyltrimethylammonium bromide ( CTAB) as precipitant and its solubility as well as antioxidant activity in vitro compared to pullulan were studied.The optimal conditions were obtained through single factor experiments and orthogonal experiments and they were as follows: the ratio of3% ( w/v) CTAB solution to initial polysaccharides solution was 1 ∶ 1, concentration of initial polysaccharides solution was7% ( w/v) , precipitation time was 12 h.Under this condition, the extraction rate of acidic polysaccharide was 5.01%.This kind of method kept structure integrity of acidic polysaccharide and pullulan and provide references for further research of this polysaccharide.Results of properties comparison between acidic polysaccharide and pullulan indicated that pullulan was easily soluble in water but acidic polysaccharide was only slightly soluble in water.The antioxidation activities of acidic polysaccharide and pullulan were similar when the concentration was in the range of 0 to 1 mg/m L. The reducing power, scavenging rate on superoxide radical and hydroxyl radical of this two kinds of polysaccharides increased with the increase of solution concentration. It could offer theoretical references for further development and application of acidic polysaccharide from A.pullulans.
- cetyltrimethyl ammonium bromide (CTAB) /
- Aureobasidium pullulans /
- acidic polysaccharide /
- pullulan /
- property

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参考文献(19)

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