Structural analysis and antioxidation activity of polysaccharide RBP-Ⅰ from Ramaria botrytoides
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摘要: 珊瑚菌经热水浸提分离得到粗多糖,纯化后得到珊瑚菌多糖RBP-Ⅰ,采用凝胶渗透色谱测定其分子量,并结合Sephadex G-100层析柱鉴定其纯度。利用苯酚-硫酸法测定糖含量,碘-碘化钾法判定是否含有淀粉,考马斯亮蓝G-250法测蛋白含量,硫酸-咔唑法测糖醛酸含量。采用红外光谱扫描、气相色谱分析其结构及单糖组成。结果表明,RBP-Ⅰ的Mw为15857 Da,为均一多糖;总糖含量86.94%,糖醛酸含量5.14%,不含蛋白及淀粉;多糖RBP-Ⅰ中单糖的摩尔比为鼠李糖∶岩藻糖∶阿拉伯糖∶甘露糖∶葡萄糖∶半乳糖=1.00∶6.53∶1.99∶8.29∶70.05∶53.80;红外光谱表明其具有多糖特征峰,且为吡喃型多糖。体外抗氧化结果表明:当多糖质量浓度为10000μg/m L时,FRAP值达到0.0672 mmol/L,DPPH自由基的清除率达到22.83%,ABTS+·的清除率达到15.71%。由此可见,珊瑚菌多糖RBP-Ⅰ具有一定的抗氧化活性。Abstract: Ramaria botrytoides RBP-Ⅰ was isolated and purified from Ramaria botrytoides through water extraction identified as homogeneous polysaccharide by gel permeation chromatography and Sephadex G-100 arbitration.The contents of total sugar, starch, protein and union acid were analyzed by phenol-sulfuric acid, law of iodine-potassium iodide, coomassie brilliant blue stain and sulfuric-carbazole method. The structure and monosaccharide composition were studied by infrared ( IR) spectra analysis and gas chromatograph.The results showed that the average molecular of RBP-Ⅰ was 15857 Da, the total sugar was86.94%, the uronic acid was 5.14% with no starch and protein.It was composed of rhamnose, fucose, arabinose, manose, glucose and galactose with a mole ratio of 1.00∶ 6.53∶ 1.99∶ 8.29∶ 70.05 ∶ 53.80.RBP-Ⅰexhibited characteristic absorption peak of polysaccharide by IR, indicating that it was pyranoid polysaccharide.Moreover, at 10000 μg/m L of RBP-Ⅰ, the FRAP value was 0.0672 mmol/L, the scavenging rate was 22.83% and 15.71% for DPPH and ABTS+· respectively. These results suggested that RBP-Ⅰhad potent antioxidant activity.
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