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中国精品科技期刊2020
杨婧娟, 何少贵, 赵梦琦, 张希. 不同方法提取绞股蓝总皂苷的比较研究[J]. 食品工业科技, 2016, (16): 269-272. DOI: 10.13386/j.issn1002-0306.2016.16.045
引用本文: 杨婧娟, 何少贵, 赵梦琦, 张希. 不同方法提取绞股蓝总皂苷的比较研究[J]. 食品工业科技, 2016, (16): 269-272. DOI: 10.13386/j.issn1002-0306.2016.16.045
YANG Jing-juan, HE Shao-gui, ZHAO Meng-qi, ZHANG Xi. Compared study on extraction of total gypenosides by different methods[J]. Science and Technology of Food Industry, 2016, (16): 269-272. DOI: 10.13386/j.issn1002-0306.2016.16.045
Citation: YANG Jing-juan, HE Shao-gui, ZHAO Meng-qi, ZHANG Xi. Compared study on extraction of total gypenosides by different methods[J]. Science and Technology of Food Industry, 2016, (16): 269-272. DOI: 10.13386/j.issn1002-0306.2016.16.045

不同方法提取绞股蓝总皂苷的比较研究

Compared study on extraction of total gypenosides by different methods

  • 摘要: 以常规乙醇回流法、酶法及微生物发酵法提取绞股蓝总皂苷,通过总皂苷含量、单体皂苷组分、抗氧化活性三方面对不同提取方法进行比较研究。结果显示,复合酶法提取所得绞股蓝总皂苷含量达7.69%,比常规回流法相对提高了31%,但对皂苷组分转化未见显著效果。微生物发酵提取破壁效果最佳,提取物中绞股蓝总皂苷含量达8.34%,比回流提取法所得含量相对提高了42.1%;经发酵处理后,绞股蓝总皂苷中活性更强的次级苷组分如绞股蓝皂苷V-AH(人参皂苷Rg3)等含量增加,对DPPH·清除的IC50值相比常规法由1.544 mg/m L降至0.562 mg/m L,对Fe3+还原力的VC当量抗氧化能力AEAC值提高至(236.299±12.785)mg VC/g固形物,显示出该方法的优越性。 

     

    Abstract: To study the extraction effects of traditional reflux techniques,enzyme techniques,microbial fermentation techniques,the content,consititude,antioxidant activities of total gypenosides were evaluated.The results exhibited that the total gypenosides contents by complex enzyme method were reached to 7.69%,Which increased by 31%comparing with traditional method,but transformation of saponins constituents did not detected signiflcantly by the enzyme techniques. The wall- breaking extraction effects of fermentation method was most excellent,the total gypenosides contents by this method were reached to 8.34%,Which increased by 42.1% comparing with traditional method,and transformation of component saponins involved in the fermentation process,such as the contents of gypenosides V- AH( ginsenoside Rg3) were increased. Due to fermentation,IC50 of DPPH radical scavenging capacity was reduced from 1.544 to 0.562 mg / m L comparing with traditional method,and ascorbic acid equivalent antioxidant capacity on reducing power of Fe3+ were reached to( 236.299 ± 12.785) mg VC/ g solids.In summary,the microbial fermentation extraction method was prior to other methods.

     

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