谷氨酰胺转氨酶和添加物对海地瓜凝胶品质的影响

何衎; 吴光斌; 陈发河

doi:10.13386/j.issn1002-0306.2016.11.005

谷氨酰胺转氨酶和添加物对海地瓜凝胶品质的影响

集美大学食品与生物工程学院

基金项目:

福建省科技计划重点项目(2013N0023)；厦门南方海洋研究中心科技项目(14GZP007NF07)；

详细信息

作者简介:
何衎(1990-),男,硕士,研究方向:水产品加工,E-mail:342512587@qq.com。;

陈发河(1960-),男,硕士,教授,研究方向:农产品贮藏与加工,E-mail:fhchen@jmu.edu.cn。;

中图分类号: TS254.1
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出版历程
- 收稿日期: 2015-11-18

Effects of transglutaminase and additive agents on gel quality of Acaudina molpadioides (Semper)

College of Food and Bioengineering,Jimei University

摘要

摘要: 本文以干海地瓜为原料,泡发后将其体壁打浆,在加热条件下与谷氨酰胺转氨酶(TGase)以及其他添加物(大豆分离蛋白(SPI)、酪蛋白酸钠(SC)和食盐)等物质进行复配,研究了体壁匀浆水分含量以及TGase、SPI、SC和食盐的添加量五个因素对海地瓜凝胶的凝胶强度、硬度、弹性和咀嚼度等品质影响。实验结果表明,SC和TGase添加量对凝胶强度影响极显著(p<0.01),其余因素对凝胶强度影响显著(p<0.05);咀嚼度和硬度受水分含量和TGase添加量影响显著(p<0.05),硬度受水分含量影响显著(p<0.05),其余因素对二者影响均不显著(p>0.05);所有因素对弹性的影响均不显著(p>0.05)。通过对比海地瓜复配凝胶和纯海地瓜凝胶发现,添加了TGase、SPI、食盐、SC等四种外源添加物的复配体系的凝胶强度、咀嚼度和硬度都有显著提升(p<0.05),而弹性的变化不显著(p>0.05)。
- 海地瓜 /
- 谷氨酰胺转氨酶 /
- 添加物 /
- 凝胶品质
Abstract: In this paper,the body wall from dried Acaudina molpadioides( Semper) after soaked and beating was used as raw material and compounded with transglutaminase( TGase) and other substances such as soy protein isolate( SPI),sodium caseinate( SC) and salt under heating conditions. Then the effects of moisture content of homogenate,addition of TGase,SPI,SC and salt on gel quality( gel strength,chewiness,hardness and springness) of the compounded system were studied.According to the results,the addition of SC and TGase had a very significant influence on gel strength( p < 0.01),and the other three factors had significant influence on gel strength( p < 0.05),chewiness was influenced significantly by moisture content and addition of TGase,and hardness was influenced significantly by moisture content( p < 0.05),while other factors showed no significantly influence on them( p > 0.05). All the factors did not have significant influence on springness( p > 0.05). With comparison between the mixed Acaudina molpadioides gel and the pure Acaudina molpadioides gel.The resualts indicated that gel strength,chewiness and hardness of the mixed system contained TGase,SPI,salt and SC were significantly increased( p < 0.05) while there was no significant influence on springiness( p > 0.05).
- Acaudina molpadioides(Semper) /
- transglutaminase /
- additives /
- gel quality

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参考文献(24)

[1]	廖玉麟.中国动物志,棘皮动物门,海参纲[M].北京:科学出版社,1997.
[2]	Yu P,Chen H.Optimization of Conditions for Enzymatic Production of Collagen Hydrolysates from a Low-Value Acaudina molpadioides and Their Activities[J].Journal of Food Biochemistry,2014,38(2):227-235.
[3]	伏纬华,吴凤梧,戚宝凤,等.海地瓜与海参营养成分的比较[J].中国现代应用药学,1991,8(3):46-47.
[4]	侯付景,李妍妍,金春华,等.白肛海地瓜和刺参体壁的比较分析[J].食品科学,2010,31(11):38-41.
[5]	蔡彬新.海地瓜多糖提取纯化及其降血脂活性研究[D].福州:福建农林大学,2009.
[6]	Yu L,Ge L,Xue C,et al.Structural Study of Fucoidan from Sea cucumber Acaudina molpadioides:A Fucoidan Containing Novel Tetrafucose Repeating Unit[J].Food Chemistry,2014,142:197-200.
[7]	王天明,苏意钢,马永钧,等.海地瓜多肽分离及抗氧化活性研究[J].现代食品科技,2014,30(5):75-81,166.
[8]	赵元晖.海地瓜蛋白水解物中ACE抑制肽的分离纯化及合成[D].青岛:中国海洋大学,2009.
[9]	Duangmal K,Taluengphol A.Effect of Protein Additives,Sodium Ascorbate,and Microbial Transglutaminase on the Texture and Colour of Red Tilapia Surimi Gel[J].International Journal of Food Science and Technology,2010,45(1):48-55.
[10]	Zhang F,Fang L,Wang C.Effects of Starches on the Textural,Rheological,and Color Properties of Surimi-beef Gels with Microbial Tranglutaminase[J].Meat Science,2013,93(3):533-537.
[11]	Cofrades S,Hughes E,Troy D J.Effects of Oat Fibre and Carrageenan on the Texture of Frankfurters Formulated with Low and High Fat[J].European Food Research and Technology,2000,211(1):19-26.
[12]	赵利,温慧芳,袁美兰,等.基于不同提取方式的鱼皮胶原蛋白对重组鱼肉品质的影响[J].现代食品科技,2015,31(3):220-227.
[13]	杨涛.北太鱿鱼碎肉蛋白质挤压组织化关键技术研究[D].舟山:浙江海洋学院,2010.
[14]	王卫芳.鱼肉猪肉复合凝胶制品的开发及其影响因素的研究[D].武汉:华中农业大学,2006.
[15]	Luo Y,Ryuji K,Masaki K,et al.Effect of Soy Protein Isolate on Gel Properties of Alaska pollock and Common Carp Surimi at Different Setting Conditions[J].Journal of the Science of Food and Agriculture,2004,84(7):663-671.
[16]	Satoshi K,Yoshiko T,Takeshi M,et al.The Effects of Salt Concentration on the Internal Macro-Structure and Texture of Walleye Pollack Surimi Gel[J].International Journal of Food Science and Technology,2006,41(4):459-463.
[17]	张怡洁,何芳,俞吉,等.带鱼纯鱼肉重组工艺以及制品蛋白质体外消化的研究[J].食品与发酵工业,2012,38(6):17-21,27.
[18]	Ramirez J,Uresti R,Tellez S,et al.Using Salt and Microbial Transglutaminase as Binding Agents in Restructured Fish Products Resembling Hams[J].Food Science,2002,67(5):1778-1784.
[19]	赵全,陈奇.酪蛋白钠稳定肉糜机理的研究[J].食品与机械,2006,22(4):52-53.
[20]	苏伟,赵利,袁美兰,等.鲢鱼纯鱼肉重组制品凝胶工艺研究[J].食品研究与开发,2013,34(3):39-43.
[21]	张云飞.鲳鱼纯鱼肉重组制品的研制及其品质特性的研究[D].杭州:浙江工商大学,2011.
[22]	孙英婷.TG酶及其他功能性添加物对豆腐肠质构特性的影响[D].南宁:广西大学,2013.
[23]	Dimitrakopoulou M A,Ambrosiadis J A,Zetou F K,et al.Effect of Salt and Transglutaminase(TG)Level and Processing Conditions on Quality Characteristics of Phosphate-free,Cooked,Restructured Pork Shoulder[J].Meat Science,2005,70(4):743-749.
[24]	安静,于国萍,初云斌,等.转谷氨酰胺酶催化对不同大豆蛋白凝胶性的影响[J].食品科学,2011,32(6):32-37.

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