Abstract: To obtain highly purified zearalenone,the mycotoxin was extracted and then purified from the fermented liquid of Fusarium graminearum in this study using different extraction solvents and chromatographic columns.The effects of extraction and purification were evaluated by ultra- performance liquid chromatography- mass spectrometry( UPLC- MS).The results demonstrated that benzene was recognized to be the best one of the single extraction agents,ewhose yield could reach up to 73.20%.Daisogel reverse phase chromatography column was much better chosen to purify zearalenone because its yield was up to 69.30% and the numbers of the impurity peaks significantly decreased.The findings of this study can provide the guidance and reference for the development of highly purity of zearalenone.