灵芝菌丝体破碎处理方法的比较

秦可欣; 石彦国; 孙冰沁; 陈浩; 何国庆

doi:10.13386/j.issn1002-0306.2016.09.020

灵芝菌丝体破碎处理方法的比较

1. 哈尔滨商业大学食品工程学院
2. 浙江大学生物系统工程与食品科学学院

基金项目:

国家自然科学基金资助项目(31271816；

详细信息

作者简介:
秦可欣(1988-),女,硕士,研究方向:大豆加工技术,E-mail:460040223@qq.com。;

何国庆(1957-),男,博士,教授,研究方向:食品生物技术及发酵工程,E-mail:gqhe@zju.edu.cn。;

中图分类号: TS219
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出版历程
- 收稿日期: 2015-10-15

Comparison crushing methods of Ganoderma lucidum Mycelium

Food Science and Engineering College,Harbin University of Commerce
College of Biosystems Engineering and Food Science,Zhejiang University

摘要

摘要: 灵芝是一种珍贵的药用真菌,含有多种生物活性物质,如灵芝多糖、三萜类化合物,同时具有较高的产β-葡萄糖苷酶活性。为了提高灵芝菌丝体中β-葡萄糖苷酶的利用效率,需要对菌丝进行破碎处理而使酶释放出来。本文研究了通过打浆处理、升温自溶、反复冻融和超声破碎的方式来提取灵芝菌丝体中的β-葡萄糖苷酶,并比较了不同方法的提取效果。结果表明,以匀浆和超声破碎结合的组合方法最佳,先在冰浴中匀浆处理1 min,在200 W下再以每超声1 min间歇1 min的方式超声破碎5次,β-葡萄糖苷酶的总活力可以达到3.116 U/m L,比直接提取提高了112.26%。显微镜观察到匀浆后菌丝球可全部分散开,同时菌丝被不同程度的切断,再经超声破碎处理,菌丝几乎全部破碎。
- 灵芝菌丝体 /
- β-葡萄糖苷酶 /
- 提取 /
- 匀浆 /
- 超声破碎
Abstract: Ganoderma lucidum is a precious medicinal fungi,which contains a variety of biological active substances,such as ganoderma lucidum polysaccharide,triterpene compounds and has high β- glucosidase activity.In order to improve the utilization efficiency of the β- glucosidase,it is necessary to break the ganoderma lucidum mycelium and release the enzyme to the full extent.Therefore,four methods of homogenate processing,heating autolysis extraction,repeated freezing- thawing,and ultrasonic crushing were used to extract theβ- glucosidase from the ganoderma lucidum mycelia,and the extraction effect of different methods was compared.The results exhibited the best extraction method for β- glucosidase extraction was the combination of homogenate processing and ultrasonic crushing.The mycelia form the fermented liquids needed to be treated by homogenate processing for 1 min in the ice bath,and then be treated 1 min intermittent 1 min for 5 times by ultrasonic crushing in 200 W.In this case,the total enzyme activity of the β- glucosidase reached 3.116 U / m L,which increased by 112.26% than that of non- broken treatment.Through microscope it can be observed that the mycelium ball was dispersed completely,the hyphae were cut off in varying degrees by homogenate and almost all were broken by ultrasonication.
- Ganoderma lucidum mycelia /
- β-glucosidase /
- extraction /
- homogenate /
- ultrasonic crushing

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参考文献(13)

[1]	Tang Ya-Jie,Zhang Wei,Liu Rui-Sang,et al.Scale-up study on the fed-batch fermentation of Ganoderma lucidum for the hyperproduction of ganoderic acid and Ganoderma polysaccharides[J].Process Biochemistry,2011,46:404-408.
[2]	Rosa'lia Rubel,Herta S.Dalla Santa,Luiz Cla'udio Fernandes,et al.Hypolipidemic and antioxidant properties of Ganoderma lucidum(Leyss:Fr)Karst used as a dietary supplement[J].World Journal of Microbiology Biotechnology,2011,27:1083-1089.
[3]	Erkel E.Iclal.Yield performance of Ganoderma lucidum(Fr.)Karst cultivation on substrates containing different protein and carbohydrate sources[J].African Journal of Agricultural Research,2009,4(11):1331-1333.
[4]	余伟,王宏勋,张晓昱.高产β-葡萄糖苷酶药用真菌菌株的筛选[J].食品科技,2008,(7):10-13.
[5]	Zhang Jingying,Ge Yinan,Han Fenxia,et al.Isoflavone content of soybean cultivars from maturity group 0 to VI grown in northern and southern China[J].Journal of the American Oil Chemists Societs,2014,91:1019-1028.
[6]	Jamie L.Mc Call,Rebekah A.Burich,Philip C.Mack.GCP,a genistein-rich compound,inhibits proliferation and induces apoptosis in lymphoma cell lines[J].Leukemia Research,2010,34:69-76.
[7]	Yuan Lan,Chihiro Wagatsuma,Sun Bu-xiang,et al.The role ofβ-glucuronidase in induction of apoptosis by genistein combined polysaccharide(GCP)in xenogeneic mice bearing human mammary cancer cells[J].Annals New York Academy of Science,2003,1010:347-349.
[8]	崔美林.高产三萜灵芝菌株液体发酵及生物转化大豆异黄酮的研究[D].杭州:浙江大学,2015:66-81.
[9]	Cui Mei-lin,He Guo-qing.Enhanced production of mycelia biomass and bioactive metabolite of the American Ganoderma lucidum in submerged culture.IEEE Xplore.2012,5:252-255.
[10]	Magdalena Klimek-Ochab,Magorzata Brzezińska-Rodak,EwaZymańczyk-Duda,et al.Comparative study of fungal cell disruption-scope and limitations of the methods[J].Folia Microbiol,2011,56:469-475.
[11]	Sebastien Jubeau,Luc Marchal,Jeremy Pruvost,et al.High pressure disruption:a two-step treatment for selective extraction of intracellular components from the microalga Porphyridium cruentum[J].J Appl Phycol,2013,25:983-989.
[12]	Vivek D.Farkade,Susan T.L.Harrison,Aniruddha B.Pandit.Improved cavitational cell disruption following p H pretreatment for the extraction ofβ-galactosidase from Kluveromyces lactis[J].Biochemical Engineering Journal,2006,31:25-30.
[13]	马腾臻,杨学山,张莉,等.红佳酿酵母β-葡萄糖苷酶活性测定及产酶特性[J].食品与发酵工业,2014,10:37-40.