Abstract: β-carotene microcapsule preparations had been prepared by high temperature melting-spray drying(HEMSD),solvent-spray-starch fluidized bed drying(SSSFBD) and wet grinding-spray-starch fluidized bed drying(WGSSFBD),and the effects of different preparation methods on its color,cis-trans isomers composition,water dispersed particle size and microstructure had been studied. Results showed that the hue of β-carotene microcapsule preparations obtained by HEMSD,SSSFBD and WGSSFBD was 60.73,41.60 and 33.57,respectively.The hue of WGSSFBD was redder,which was suitable for coloration and fortification of red series of food and beverages. The hue of HEMSD was yellower,but the hue of SSSFBD was between in the red and yellow,there were more suitable for coloration and fortification of yellow to orange series of food and beverages. β-carotene microcapsule prepared by HEMSD had the highest proportion of cis isomers and its water dispersed particle size was the largest,while the proportion of cis isomers of β-carotene microcapsule prepared by WGSSFBD was the lowest,which was 21.6% and 1.7%,respectively. β-carotene microcapsule prepared by HEMSD was spherical microstructure,dense surface and no cracking,but its microstructure was similar bayberry shape when obtained by SSSFBD and WGSSFBD,and its particle size was relatively larger.