Abstract: A novel slow release hydrogel was prepared by bovine serum albumin(BSA) and citrus peel pectin via self-assembly. The significant influence on the diameter of hydrogel was found and discussed by three factors Mass ratio(MR) of pectin and BSA,protein concentration,and p H at three level in the preparation of the hydrogel. Combined with the characterization of rheology,CD and SEM,the paper further focused on investigating the effective factor MR of CPP and BSA. The results showed that mixture of pectin and BSA reached the smallest particle size 259.5 nm with a narrow size distribution(PDI<0.06) and formed a good and stable hydrogel with three-dimensional network structure when heated with mass ratio(MR) of CPP:BSA 0.5 at p H4.5. The result indicated that the hydrogels(BCH) had a high encapsulation efficiency(EE,49.88%) and slow release under simulated gastric and intestinal fluid condition when salidroside was used as a model molecule,which showed that this novel hydrogel might be a potential deliver carrier for salidroside or other functional food agents.