Abstract: In this paper,binding force between mesoporous materials and trypsin was improved by introducing different modifiers functional groups on the surface of SBA-15(- SH,- Cl,- NH₂,-C₁,-C₃,-C₈ and- C₁₆) in order to increase the efficiency of enzyme loading,activity and stability.Activity of PT- NH₂/ C₈- SBA could be found up to255 U / mg trypsin,the activity of PT- SBA was just 195 U / mg trypsin. After the fifth recycle and storage at room temprature of 5 d,PT- NH₂/ C₈- SBA was still able to maintain 85% and 81% of initial activity,while the PT- SBA retained only 51% and 67% of the initial activity.Kinetic studies showed that immobilized enzyme PT- NH₂/ C₈- SBA has better affinity with substrate.