一种蛹虫草多糖的结构特征及体外抗氧化活性

Structural characterization of a polysaccharide from cultured Cordyceps militaris with antioxidant activity

摘要: 采用分子筛层析法从人工培养蛹虫草子实体中分离纯化得到一种多糖W-CBP80Ⅰ,进而分别采用凝胶渗透色谱（GPC）、气-质联用（GC-MS）、红外光谱（IR）、核磁共振(¹H NMR和¹³C NMR)研究其理化性质和结构特征。结果表明:W-CBP80Ⅰ含有α-糖苷键,并主要由甘露糖、半乳糖和葡萄糖组成。它主要成分为低分子量多糖,分子量为8.93×10³。此外,W-CBP80Ⅰ具有体外清除DPPH,羟基和超氧阴离子自由基活性。

Abstract: A polysaccharide W-CBP80 Ⅰ was isolated from the fruiting bodies of cultured Cordyceps militaris and characterized using GPC,GC-MS,IR,~1H NMR and ¹³C NMR. The results indicated that it contained α-type glycosidic linkages and was mainly composed of α-mannose,α-galactose and α-glucose. Its main component was low molecular weight polysaccharide with Mwof 8.93×10~3. In addition,W-CBP80Ⅰ exhibited scavenging capacities against DPPH,hydroxyl and superoxide radicals in the antioxidant assay in vitro.