Abstract: Objective:The content of oleanolic acid(OA) and ursolic acid(UA) in different arts(roots,stems,fruits)of Rosa Multiflora Thunb. have been compared,and taken analysis on content of OA and UA in Rosa Multiflora Thunb. fruit from different origin by HPLC. Methods :Zorbax ODS chromatography column(4.6 mm×250 mm×5 μm),methanol-water(88∶12) was used as mobile phase,diode array detector at 210 nm wavelength had been used for detection. Results:The OA and UA was better separated within 20 minutes,it had good linear relationship between peak area mass concentration between 0.02468~0.2468 mg/m L and 0.02523~0.2523 mg/m L with the correlation coefficient of 0.9994 and 0.9996 respectively. The recovery rates were 99.96%(RSD 0.74%)and 100.03%(RSD 0.95%). Conclusion:The established method was simple,quick,accurate,and had better reproducibility for the determination of OA and UA in Rosa Multiflora Thunb. at the same time by HPLC. The content of OA and UA in fruits reached the highest value. The Rosa Multiflora Thunb. from Kuqa and Atux had the highest content of OA and UA,so that could be used as the origin for the research of Rosa Multiflora Thunb.