Abstract:
Total flavonoids from Siberian apricot flowers were extracted with the method of flash extraction and purified with X-5 macroporous resins.The antioxidant activity of separated and unseparated total flavonoids from Siberian apricot flowers were investigated by measuring scavenging effect on DPPH·and OH·,total reduction capacity,total antioxidant capacity and the inhibitory effect on tyrosinase activity was studied by measuring the rate of tyrosinase catalyzed L- DOPA oxidation taking VCand β- arbutin as positive controls. The results showed that separated and unseparated total flavonoids from Siberian apricot flowers had a certain antioxidant activity.After separation,the antioxidant activity was improved obviously,the inhibitory effect on tyrosinase of separated and unseparated total flavonoids from Siberian apricot flowers was better than β- arbutin but worse than V
C. Their half maximal inhibitory concentration( IC
50) were 2.24,3.08 mg /m L,respectively. In conclusion,separated and unseparated total flavonoids from Siberian apricot flowers had a certain antioxidant activity and can effectively inhibit tyrosinase activity.