Abstract: In order to improve the bile salt hydrolase production of Lactobacillus plantarum KLDS1.0386,the response surface method was used to optimize the medium composition. The optimal values of the culture medium were as follows(g/L) :glucose 18.2 g/L,peptone 15.03 g/L,yeast extract 9.97 g/L,sodium acetate3.13 g/L,diammonium hydrogen citrate 2.00 g/L,K_2HPO_4 2.00 g/L,Mn SO_40.25 g/L,Mg SO_4 0.58 g/L. Before optimizing,the activity of bile salt hydrolase was 1.04 U/mg,Under the optimal conditions,specific enzyme activity of bile salt hydrolase was 3.37 U/mg,increasing 3.24 times than before. The experimental result was consistent with the value predicted by the mathematical model. which showed the model can be put into use.