Abstract: Preparation hemin with high content hemoglobin in yak blood.With fresh yak blood as the material to get the hemoglobin through pre- treatment,then through enzymolysis to get the hemin.On the basement of single factor test,using combination Box-Benhnken center rotation experiment and response surface analysis,the best extraction process of hemin from hemoglobin by enzymolysis as follows: 55 ℃ for enzymolysis temperature,94 min and p H8.0for enzymolysis,enzyme concentration was 0.08 g. After optimization,the yield of hemin reached 6.47 μg / m L. The enzymolysis yak blood by papain extract hemin is a useful method.