Abstract: The objective of this study was to investigate the content of carbonyl,sulfhydryl,bityrosine,the surface hydrophobicity,the turbidity and emulsifying of the oratosquilla oratoria salt-soluble protein treated by hydroxyl radical generating systems containing 0.01 mmol/L ferric trichloride,0.1 mmol/L ascorbic acid and 0~20 mmol/L hydrogen peroxide for 1,3 h and 5 h respectively. The results revealed that the carbonyl content,the bityrosine content,the turbidity and the sulfhydryl increased,while sulfhydryl content,emulsifying activity and emulsion stability decreased. These oxidation-induced structural changes revealed high susceptibility of the oratosquilla oratoria salt-soluble protein to oxidative stress.