Abstract: Different parts of Ixeris Chinensis( Thunb.) Nakai( root,stem,leaf and flower) were extracted with 70%ethanol using ultrasonic extraction for 30 min. The contents of polysaccharide,flavonoids,phenolic acid and saponins extracted from four different parts were determined respectively. The antioxidant activity of extracts and the effect of acetylcholine content on nicotine acute poisoning in mice were investigated. The results showed that the difference was obvious among the nutritious components of different parts of Ixeris Chinensis. The order of content of polysaccharide and flavonoids: root > leaf > flower > stem; the order of content of saponins and phenolic acid: leaf > flower > stem > root. The order of the antioxidant activity of the extracts: leaf > flower > stem > root. It sugessted that the antioxidant activity was mainly associated with flavonoid,phenolic acids,and the extracts had both certain inhibition to the acetylcholine on nicotine acute poisoning in mice.The results provided the theoretical base for the development of Ixeris Chinensis.