淫羊藿药渣中多糖的提取及其体外抗氧化活性评价

Polysaccharides extraction from residue of Epimedium and its antioxidant activity evaluation

摘要: 目的:从淫羊藿药渣中提取多糖,考察其得率与体外抗氧化活性。方法:采用水煮法从提过总黄酮的淫羊藿药渣中提取多糖,与其他直接采用淫羊藿原药材提取多糖的工艺进行得率和体外抗氧化活性的比较,体外抗氧化活性从对DPPH·、O-2·和·OH的清除率三方面评价。结果:从药渣中提取多糖的得率为3.68%,DPPH·、O-2·和·OH的IC50分别为0.262、0.025、0.755 mg/m L。结论:淫羊藿药渣中提取多糖的工艺操作简单、能耗低,所得多糖损失少、体外抗氧化活性强。

Abstract: Objective: To extract polysaccharides from residue of Epimedium,explore its yield and antioxidant activity in vitro.Method: Polysaccharides were extracted by boiling- water method from residue of Epimedium which the total flavones had been extracted,the yield and antioxidant activity in vitro were compared with some other different extraction methods using crude materials of Epimedium.Antioxidant activity in vitro was evaluated by the scavenging rates to 3 kinds of free radicals,DPPH·,O-2·and ·OH. Result: The yield of the polysaccharides were extracted from residue was 3.68%.IC50 of DPPH·,O-2·and·OH were respectively 0.262,0.025,which 0.755 mg /m L.Conclusion: This technology not only consumed low- power,operated simply but also had a low loss and the polysaccharides from the residue had strong antioxidant activity in vitro.