Abstract: For the sake of developing gelatin-calcium lactate composite films with anti-microbial activity,different concentration of wine wash was added to it. In addition,the mechanical properties,optical properties,surface topography,anti-microbial activity and preservation effect of wine wash-gelatin-calcium lactate were also investigated and analyzed. The results indicated that the incorporation of wine wash caused interactions between gelatin and ingredients in wine wash,and the films showed increased thickness and elongation. After the accretion of wine wash,the films showed darker appearance,and decreased tensile strength,water solubility and water vapor permeability. Meanwhile,the inhibition to food spoilage microorganisms and preservation effect enhanced,and the surfaces became rougher by AFM with the increase of concentration of wine wash. In summary,gelatin-calcium lactate composite films incorporated with wine wash presented application capacity in food packaging and preservation.