紫外线与微波复合诱变选育高产脂肪酶菌株的研究

Research of UV/microwave irradiation for breeding of a high lipase-producing strain

摘要: 研究选育高产脂肪酶菌株。采用紫外线诱变、微波诱变和紫外微波复合诱变出发菌株H3,测定比较酶活,确定最佳诱变效果。结果表明,出发菌株经紫外线和微波诱变后,所产脂肪酶的最高酶活分别为57.984 U/m L和57.1 U/m L,比出发菌株H3提高了38.3%和36.2%,经微波辐射40 s和紫外线照射80 s的复合诱变菌株M3产酶活力最高,达61.6 U/m L,比出发菌株H3提高了46.85%。

Abstract: Breeding of a high lipase- producing strain was studied. The original strain H3 was treated by UV mutation, microwave mutation and compound mutation of 40 s UV and 80 s microwave, the optimum effect was determined by comparing with the enzyme activity.The result showed that the enzyme activity of the strain treated by UV mutation and microwave was 57.984 U / m L and 57.1 U / m L. The enzyme activity was improved 38.3% and36.2% than H3. The enzyme activity of the stain treated by compound mutation was 61.6 U / m L, it was improved46.85% than H3.