Abstract: To explore the relationship between melanin and Auricularia auricular, according to existing research, taking methanol as solvent, the sort of compound in extraction was identified by TLC etc.B16 melanoma cells were used as indicator cells, melanin content was determined by measuring the absorbance at 405 nm.Cell viability or cytotoxicity effect was assayed by determining the number of living cell using MTT assay and comparing with arbutin.The results showed that Auricularia auricular extract had polyphenols, terpenoids, polysaccharides and amino acids, and it had strong inhibitory effect on melanin biosynthesis and had low cytotoxicity to B16 melanoma cells when the concentration was high.