Abstract: For further research on the main pathogenic bacteria of postharvest sweet cherry fruit and its control technology,sweet cherry(cv.Labinsi) as test material,the pathogenic bacteria of sweet cherries was isolated,identified in low temperature storage,antifungal activity of plant essential oil and its treatment were studied.According to the characters of pathogen morphology and sequences analysis of 18 S r DNA,the pathogenic bacteria was Mucor sp. and Colletotrichum gloeosporioides. thyme and clove oil showed complete inhibitory effect on all pathogens. The treatment of thyme oil had no effect on the growth of Mucor sp. and Colletotrichum gloeosporioides. However fumigation treatment showed strong antifungal activities against the treatment of contact to Colletotrichum gloeosporioides. The MBC of thyme oil against these two pathogens were 2000 and1000 μL/L,respectively. Compared with fumigation,contact treatment of clove oil was better and the MBC of clove oil against these two pathogens were 500 and 1000 μL/L,respectively.