水溶性黑木耳多糖体外结合胆酸盐能力的分析

In vitro binding of bile salts by water- soluble AAP

摘要: 本文通过水提醇沉提取黑木耳多糖,同时用除蛋白和不除蛋白两种方法处理,得到12种不同浓度醇沉水溶性混合多糖;并对12种多糖的体外结合胆酸盐能力进行分析。结果显示:70%乙醇醇沉得到的水溶性黑木耳多糖体外结合胆酸作用较强,并且除蛋白多糖比不除蛋白多糖体外结合甘氨酸胆酸钠的能力强。同时,多糖浓度0.8 mg/m L时,结合达到稳定,为最适宜加入量。实验结果为AAP在降血脂机制方面的进一步研究提供理论依据。

Abstract: 12 kinds of water- soluble polysaccharides were obtained by water extraction and deposited with different concentration of ethanol under condition of removing protein or not in this paper. The results showed: the water-soluble polysaccharides deposited by 70% ethanol had stronger binding capability.Also,AAP removed protein had a stronger ability of binding glycine cholate than the unremoved group.Meanwhile,when the concentration of AAP was 0.8 mg / m L,the combination was stable,for the most appropriate amount. And the experiment provides a theoretical basis for further study on reducing blood lipid function of AAP.