Abstract: Strains from ice fresh chicken were isolated with selective culture medium,identified with 16 S r RNA gene sequencing method,and analyzed the production of biogenic amines with thin layer chromatography and HPLC.Results indicated that 11 strain species were found from chilled chicken,named intestinal C1,C4,C7,C8,PCA1,PCA2,PCA3,PCA4,PCA5,PCA7 and PCA8.PCA1 was identified as Stenotrophomonas,PCA4 was Kocuria,PCA3 was Enterobacter asburiae,PCA7 was Enterobacter cancerogenus,C1 was Enterobacter ludwigii,C4 was Enterobacter cloacae,C7 was Enterobacter hormaechei,C8 was Enterobacter hormaechei,PCA2 was Kocuria rhizophila,PCA5 and PCA8 was Staphylococcus. Among them,except PCA2,PCA5 and PCA8,all strains could produce biogenic amines by thin layer chromatography.The biogenic amine types and content produced by strains was further evaluated with HPLC,and it was found that PCA7 produced putrescine only 844.937 μg / m L; the other strains product various contents of putrescine and cadaverine.In conclusion,ice fresh chicken susceptible to intestinal bacteria pollution,and produces putrescine and cadaverine.This study also can be used as according in the other research about ice fresh chiken.