室温下麦粒中脱氧雪腐镰刀菌烯醇(DON)及其隐蔽型(D3G)毒素累积分析

靳梦曈; 王建华; 林善海; 赵勇; 孙文烁; 武爱波

doi:10.13386/j.issn1002-0306.2015.17.018

室温下麦粒中脱氧雪腐镰刀菌烯醇(DON)及其隐蔽型(D3G)毒素累积分析

1. 上海海洋大学食品学院
2. 上海市农业科学院农产品质量标准与检测技术研究所

基金项目:

国家自然科学基金(31201378;31401598)；

详细信息

作者简介:
靳梦曈(1989-),女,硕士研究生,研究方向:食品科学与工程,E-mail:m13162737696@163.com。;

武爱波(1977-),男,博士,研究员,研究方向:真菌毒素检测安全评价与控制,E-mail:wuaibo2007@aliyun.com。;

中图分类号: TS210.1
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出版历程
- 收稿日期: 2014-11-23

Analysis of deoxynivalenol( DON) and masked deoxynivalenol( D3G)accumulation in different treatment wheat grains at room temperature

College of Food Science and Technology,Shanghai Ocean University
Institute for Agro- food Standards and Testing Technology,Shanghai Academy of Agricultural Sciences

摘要

摘要: 为了研究麦粒中脱氧雪腐镰刀菌烯醇（DON）及其隐蔽型脱氧雪腐镰刀菌烯醇-3-葡萄糖苷（D3G）的累积情况,将禾谷镰刀菌菌株F-1和5035分别人工接种至经高温灭菌、8 k Gy和15 k Gy辐照的麦粒中25℃培养7、14、21、28、35 d后,通过LC-MS/MS方法测定DON和D3G毒素在麦粒中的含量。不同处理的麦粒中DON含量均随培养时间延长逐渐增加;禾谷镰刀菌接种灭菌麦粒及15 k Gy辐照麦粒均未检出D3G毒素;禾谷镰刀菌接种8 k Gy辐照小麦籽粒后检测到隐蔽型毒素D3G,培养7 d时D3G最高且其含量随着培养时间延长逐渐降低;本文使用指数模型拟合25℃下F-1与5035接种麦粒（8 k Gy辐照）中D3G/DON的比值与时间关系的R2分别为0.97、0.93,R2均在0.9以上,说明指数模型能够较好地拟合本实验条件下时间与D3G/DON的比值的变化,为进一步后续研究提供依据。
- 小麦籽粒 /
- 禾谷镰刀菌 /
- DON /
- D3G
Abstract: To elucidate the content of deoxynivalenol( DON) and masked deoxynivalenol( D3G) accumulation and variation in wheat grain. The sterilized wheat grain,with 8 k Gy and 15 k Gy irradiated wheat grain inoculated artificially with Fusarium graminearum strains F-1 and 5035 respectively,were determined by LC- MS / MS after 7,14,21,28 and 35 days of incubation under 25 ℃. The results showed that DON content increased gradually with time in sterilized and 15 k Gy irradiated wheat grain while the content of D3 G were blow limit of detection.The wheat grains can germinate after 8 k Gy irradiation,DON content increased gradually with time in 8 k Gy irradiated wheat grain while the content of D3 G decreased with time. The highest D3 G content was detected in wheat grain incubated after 7 days.The relationship between the ratio of D3 G / DON and incubation time can be well described by exponential model in the conditions of this experiment. R2 of F- 1 and 5035 exponential models regarding the ratio of D3 G / DON and time were 0.97 and 0.93,respectively.As convinced,our results could provide the basis for subsequent studies on the regulatory mechanism of D3 G biosynthesis.
- wheat grain /
- Fusarium graminearum /
- DON /
- D3G

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