Abstract: This study was to investigate the prophylactic effect of microencapsulation kiwi fruit on activated carboninduced constipation in Kunming mice. KM mice were fed with microencapsulation kiwi fruit for 9 d. Body weight,defecation status,defecation time,gastrointestinal transit,and the serum levels of MTL( motilin),Gas( gastrin),ET( endothelin),SS( somatostatin),ACh E( acetylcholinesterase),SP( substance P),VIP( vasoactive intestinal peptide) were used to evaluate the preventive effect of microencapsulation kiwi fruit on constipation. Bisacodyl,a laxative drug,was used as a positive control. After inducing constipation,the defecation status of mice fed with microencapsulation kiwi fruit was almost normal,was close to the normal mice and constipation therapeutic drug( bisacodyl) treated mice.10% microencapsulation kiwi fruit could reduce the time to the first black stool defecation( 112 min),which was shorter than 5% microencapsulation kiwi fruit treated mice.10% microencapsulation kiwi fruit could also increase the gastrointestinal transit 77.7%,which was significantly higher than 5% microencapsulation kiwi fruit and control group. In contrast to the control mice,the serum levels of MTL,Gas,ET,ACh E,SP and VIP were significantly( p < 0.05) increased and the serum levels of SS were reduced in the mice treated with microencapsulation kiwi fruit.Through the analysis of components,microencapsulation kiwi was rich in vitamin A,C,E and dietary fiber.These results demonstrated that microencapsulation kiwi included rich functional components,and it had preventive effect on mouse constipation.