温度及乳酸链球菌素对单增李斯特菌的抑制作用

姜爱丽; 胡文忠; 崔晓亭; 陈晨; 萨仁高娃; 李佳慧

doi:10.13386/j.issn1002-0306.2015.15.025

温度及乳酸链球菌素对单增李斯特菌的抑制作用

1. 大连民族大学生命科学学院
2. 大连理工大学生命科学与技术学院

基金项目:

国家科技支撑计划项目(2012BAD38B05)；国家自然科学基金资助项目(31172009)；中央高校基本科研业务费项目(DC2013010107)；辽宁省科技厅基金；

详细信息

作者简介:
姜爱丽(1971-),女,博士,副教授,研究方向:采后生物学与技术,E-mail:jal@dlnu.edu.cn。;

胡文忠(1959-),男,博士,教授,研究方向:食品科学与工程,E-mail:hwz@dlnu.edu.cn。;

中图分类号: TS201.3
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出版历程
- 收稿日期: 2014-10-20

Inhibition of temperature and Nisin on Listeria monocytogenes

College of Life Science,Dalian Nationalities University
College of Life Science and Biotechnology,Dalian University of Technology

摘要

摘要: 通过Matlab软件拟合Gompertz生长模型,研究了温度对单增李斯特菌(Listeria.monocytogenes,LM)的影响;同时,研究了不同浓度(5、10、50、100、150μg/m L)、p H(1、3、5、7、9)、温度(45、75、95、115、121℃)条件下的乳酸链球菌素对LM杀菌活性的影响,旨在为LM的监控技术发展提供理论依据。结果表明:低温的抑制效果明显,最大细胞密度减少了4.3455 lg cfu/m L;乳酸链球菌素浓度低于5μg/m L时能促进LM的生长,高于10μg/m L时,对LM有一定的杀菌作用,高于150μg/m L时,48 h之内几乎可以杀死营养肉汤中的所有LM;乳酸链球菌素对酸性有协同效应;并有较好的热稳定性。
- 单增李斯特菌 /
- 生长模型 /
- 乳酸链球菌素 /
- 温度 /
- p H
Abstract: The effect of different temperatures on growth of Listeria monocytogenes( LM) was studied,and Gompertz model was fit using Matlab software. In order to provide theory foundation for the development of supervisory technique,the antibacterial activity of Nisin under the different concentrations( 5,10,50,100,150 μg / m L),p H( 1,3,5,7,9) and temperatures( 45,75,95,115,121 ℃) were studied simultaneously. The results demonstrated that low temperature appeared significantly inhibiting effect,the maximum cell density was reduced by 4.3455 lg cfu / m L. The growth of LM was promoted when the concentration of Nisin was < 5 μg / m L. The bactericidal effects was the concentration of Nisin > 10 μg / m L.When the concentration of Nisin was > 150 μg / m L,it was able to kill almost all L. monocytogenes in nutritional broth during the period of 48 h cultivation. Nisin has a synergistic effect under acidic environment and a good thermal stability.
- Listeria monocytogenes /
- growth model /
- Nisin /
- temperature /
- p H

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参考文献(14)

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