薛氏丙酸杆菌产抑菌性代谢物发酵培养基优化

Optimization research of fermentation mediums for antimicrobial metabolites producing strain Propionibacterium shermanii

摘要: 本文利用单因子实验和响应面实验设计对薛氏丙酸杆菌产抑菌性代谢物的发酵培养基组成进行优化,以降低培养基成本和进一步提高代谢物抑菌活性。实验结果表明,玉米浆和大豆蛋白胨可以代替原始培养基中的酵母提取物和胰蛋白胨,通过Box-Behnken实验设计和响应面分析法优化得到了薛氏丙酸杆菌产抑菌性代谢物的发酵培养基组成为葡萄糖8.2g/L,大豆蛋白胨5.1g/L,玉米浆12.7g/L,在此条件下,代谢物抑菌活性的理论值为29.5AU/m L,实际测定值为29.4AU/m L。优化之后,薛氏丙酸杆菌代谢物抑菌性提高了57.2%,培养基成本大大降低。

Abstract: The fermentation medium was optimized to reduce the medium cost and promote the antimicrobial metabolites production of Propionibacterium shermanii by simple factor experiment and response surface methodology.The results showed that the yeast extract and tryptone of original medium could be replaced by the corn steep liquor power and soy peptone.According to Box- Behnken design and response surface methodology, the optimal medium were as follows: glucose 8.2g / L, soy peptone 5.1g / L, and corn steep liquor 12.7g / L. After medium optimization, the theory value of antimicrobial activity was 29.5AU / m L, and was improved by 57.2%. The actual operation of the experiment turned out to be 29.4 AU / m L, and the medium cost was reduced.