Abstract: The fresh strawberry ( Fragaria ananassa Duch. cv. Hongshimei) was used as the experimental material and different concentrations of essential oil from Longan seed was employed to study on the preservation effects of the post harvest strawberry under low temperature condition ( 4℃) . The results showed that the essential oil from Longan seed had remarkable effects on the preservation of strawberry, which could reduce the decay index and slow down the losing speed of soluble solid, total sugar, vitamin C, total flavone and phenolics effectively, the radical scavenging ability against DPPH and ABTS of the strawberry was improved and its antioxidant activity was kept.The strawberry treated by the essential oil with the concentration of 160μg / m L showed the best effect, all of indexes but titrable acidity were superior to other treatments and control group remarkably.