Abstract: Six different extraction methods,including water extraction,enzymatic extraction,ultrasonic method,ultrasonic- enzymatic method,microwave method and microwave- enzyme method,were used to extract Cordyceps polysaccharide from Cordyceps militaris culture medium.The extraction yield,viscosity and the impact of antioxidation were evaluated to compare the advantage and disadvantage of different extraction methods. The results showed that polysaccharide prepared by ultrasonic- enzymatic extraction showed the highest extraction yield 30.27%,while that of water extraction showed the lowest yield 10.69% at the same time. Polysaccharide viscosity with the increase of polysaccharide solution concentration by different extraction methods,the apparent viscosity at the same concentration was: water extraction > microwave extraction > enzyme extraction > microwave- enzymatic extraction > ultrasonic extraction > ultrasonic- enzymatic extraction. The polysaccharide prepared by ultrasonic- enzymatic extraction exhibited the strongest antioxidant activity,while that of water extraction showed the lowest antioxidant activity.