Abstract: According to the different dissolve ability of protein components, four kinds of protein components had got from turtle protein. In order to evaluate the nutritional value,components of each protein fraction and composition of amino acids were analyzed. The result showed that the content characteristics of protein components was glutelin >albumin >globulin >prolamine. Glutelin was the predominant component in turtle protein. Seventeen common amino acids were found in Turtle protein and four kinds of protein components.The ratio of total essential amino acids to total amino acids,and the ratio of total essential amino acids to total nonessential amino acids were higher than(FAO/WHO) standard. Their limited amino acid was Met +Cys.Glutelin and globulin possessed higher AAS,CS,EAAI,and PERⅠ value,with good nutritional quality.