Abstract: Studied the best condition for the extraction of the red pigment from the Crataegus jingxiensis Li peel through microwave-assisted technology,and investigated the influence of alcohol concentration,liquid ratio,microwave power,microwave minute on the extraction of the pigment. In the whole process of the experiment,the above conditions were optimized by the orthogonal experiment. Antibacterial activities of red pigment were determined by paper disk diffusion. Results showed that the best conditions for the extraction of the red pigment from the Crataegus jingxiensis Li peel were as follows:alcohol concentration 60%,liquid ratio 1∶50(g/m L),microwave power 500 W,microwave minute 60 s and extraction twice. The results of the antibacterial experiments showed that the red pigment had certain inhibitory effect on Staphylococcus aureus, Staphylococcus epidermidis,Escherichia coli and Salmonella enterica.