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中国精品科技期刊2020
陈锦, 胡茂丰, 吴章华, 刘素纯. 高产阿魏酸酯酶菌株的筛选及其产酶条件优化[J]. 食品工业科技, 2015, (08): 218-221. DOI: 10.13386/j.issn1002-0306.2015.08.037
引用本文: 陈锦, 胡茂丰, 吴章华, 刘素纯. 高产阿魏酸酯酶菌株的筛选及其产酶条件优化[J]. 食品工业科技, 2015, (08): 218-221. DOI: 10.13386/j.issn1002-0306.2015.08.037
CHEN Jin, HU Mao-feng, WU Zhang-hua, LIU Su-chun. Selection of high-yield ferulic acid esterase strain and optimization of enzyme production conditions[J]. Science and Technology of Food Industry, 2015, (08): 218-221. DOI: 10.13386/j.issn1002-0306.2015.08.037
Citation: CHEN Jin, HU Mao-feng, WU Zhang-hua, LIU Su-chun. Selection of high-yield ferulic acid esterase strain and optimization of enzyme production conditions[J]. Science and Technology of Food Industry, 2015, (08): 218-221. DOI: 10.13386/j.issn1002-0306.2015.08.037

高产阿魏酸酯酶菌株的筛选及其产酶条件优化

Selection of high-yield ferulic acid esterase strain and optimization of enzyme production conditions

  • 摘要: 通过初筛、复筛从茯砖茶中筛选到了1株产阿魏酸酯酶活力较高的菌株,经个体形态和菌落特征初步鉴定其为冠突散囊菌;以阿魏酸酯酶酶活为衡量指标,选取黑毛茶含水量、接种量、培养温度、培养时间为因子,研究确定筛选出来的冠突散囊菌进行固态发酵黑毛茶的产酶条件。结果表明:黑毛茶固态发酵实验中,黑毛茶含水量26%,接种量1‰,温度30℃条件下培养4d,其酶活力最高可以达到142m U/g。 

     

    Abstract: Through preliminary and secondary screening,a strains with high enzyme activity which could produce feruloyl esterase was screened out from Fu-brick tea,according to its individual and colony characteristics,it was identified as Eurotium cristatum. Feruloyl esterase enzyme activity was used as measurable indicator,water content,inoculums-size,temperature and time was chosen as variables,the solid-state fermentation conditions of chosen Eurotium cristatum were optimized. The results showed that black primarytea the optimal condition was 1‰ inoculums size,26% moisture content and cultivated at 30℃ for 4 days,the highest enzyme activity reached 142 m U/g in the black Maocha solid-state fermentation experiment.

     

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