Abstract: A dispersive solid-phase extraction-gas chromatography(GC) method had been developed for the detection of six kinds of eugenol drug residues in aquatic products at the same time. Sample was ultrasonic extracted by acetone,constant volume after rotary evaporation concentration,using dispersive solid-phase extraction(DSPE) purification,determined by gas chromatograph. External standard calibration was adopted in the determination. Six kinds of eugenol type of anesthetic showed good linearity in the concentrations range of0.05~10.0μg/m L for the standard solutions carves,and all of the correlation coefficents(R2) were in 0.9995~0.9999. At the spiked levels of 0.1~1.0mg/kg in tilapia,south America white shrimp,eel,swimming crab muscle,the average recoveries were 80.3% ~103.2%. The relative standard deviations were 0.7% ~5.4%. The limits of quantificationg(LOQ),all of the six kinds of eugenol drug residues(Eugenol,Methyl eugenol,Iso-eugenol,Methyl iso-eugenol,Eugenol acetate,Acetyl iso-eugenol)were 0.1mg/kg.