Abstract: A method was developed for determination of the migration of epoxidized soybean oil( ESBO) from PVC gaskets of glass jars in food simulant by gas chromatography- mass spectrometry( GC- MS). The gaskets were exposured to distilled water and olive oil at 40℃ for 240 h,and ESBO was treated by methyl esterification and derivatization.Ethyl 11,14- diepoxyeicosanoate was used as internal standard to determine the epoxy linoleic acid in ESBO for ESBO quantification by GC- MS.The results showed the calibration curves of ESBO was linear in the range of 0.5 ~ 50 mg / kg with correlation coefficients not less than 0.993. The spiked recoveries at different spiked levels were in the range of 92.8% ~ 103.9% with relative standard deviations( RSD) of 0.9% ~ 8.0%. The limit of detection( LOD) was 0.2mg / kg and the limit of quantitation( LOQ) was 0.5mg / kg. This method was precise,highly sensitive and accurate,which was applied in determination of the migration of ESBO in food simulants for 24 PVC gaskets.The results showed that the migration of ESBO in distilled water was lower than the regulations of EC10 /2011,but its content was between 0.7~501.8mg / kg and 7 of them exceeded the regulations in olive oil.