分散固相萃取高效液相色谱法测定谷物中的桔霉素

Determination of citrinin in grain by dispersive solid- phase extraction and HPLC

摘要: 目的:建立谷物中桔霉素含量的测定方法。方法:以60%乙醇作提取剂,C18吸附剂分散固相萃取净化样品中的桔霉素,应用高效液相色谱进行分析。结果:该方法标准曲线方程Y=3.13×10-7X-5.59×10-2,线性相关系数R2=0.9999,检出限为0.005mg/L,重复性实验精密度（RSD）为4.51%,加标回收率范围为87.7%⁹6.4%,所测的三种谷物样品桔霉素含量为0.116⁰.385μg/g。结论:该方法简便、准确,可用于谷物中桔霉素含量的测定。

Abstract: Objective: To establish a method to determine the content of citrinin in grain. Method: The samples were extracted with 60% ethanol,and cleaned up by dispersive solid- phase extraction with C18 dispersant sorbent and ready for HPLC determination.Results: A standard curve equation was built as follows: Y = 3.13 × 10- 7X-5.59 × 10- 2with linear correlation coefficient of 0.9999 and RSD was 4.51%.The average recovery range was 87.7% ~96.4%.The citrinin content range of the samples was 0.116 ~ 0.385μg / g.Conclusion: This method was sample and accurate,which could be used for detecting citrinin in grain.