Abstract: The physicochemical properties,fatty components,contents of minerals and contents of functional materials had been mainly researched with crab oil using gas chromatography, flame atomic absorption spectrometry and ultraviolet spectrophotometry. The antioxidant activity of crab oil was also analyzed. The results showed the content of UFA of crab oil was 76.79%,higher than SFA. Among them,the contents of palmitic acid,oleic acid,linoleic acid,EPA,DHA were 17.09%,33.92%,20.20%,3.73% and 4.19%,respectively.The content of P, Na, K, Ca were rich. The contents of astaxanthin and phospholipids were 0. 051 % and118. 28 mg / g, respectively. And the antioxidant activity of crab oil increased with the increasing of mass concentration. It indicated that crab oil had the high nutritional value and could be developed as a functional food and had promising application potential.