Abstract: In this study,the toxicity of the brevibacterium wettable powder(WP) on major postharvest pathogens of citrus and their efficacy in preserving the quality of glorious orange were investigated. The results showed that brevibacterium WP had the stronger toxic effects on Penicillium italicum Wehmer and Penicillium digitatum Saccardo,EC50 value were 7.4970μg/m L and 8.8832μg/m L,according to preservation effect of view. The results showed that there were significant control effect of the brevibacterium WP 2000 times against Penicillium,green mold,anthracnose and total diseases,in 60 days. The brevibacterium WP had the better control effect than the control agent prochloraz EC at 667 times,comparison with water control. In 15 to 45 days the 100 billion/brevibacterium WP dip drug treatment had no effect on the weight loss of Glorious orange. After 60 day the brevibacterium WP had some influence on fruit weight loss Glorious orange. The brevibacterium 2000 times and the water had no difference on fruit nutritional quality impact,but no negative impact on the appearance,color and taste of the Glorious orange fruit. Sum up:the brevibacterium WP 2000 times had better preservation effect of fruit storage to glorious orange.