Abstract: The optimum preparation process of antioxidant peptide from glutinous rice bran and its free radical scavenging capability were studied to exploit and utilize the resources of glutinous rice bran. Neutral protease was selected as hydrolytic enzyme,the single-factor experiment and the orthogonal tests were carried to optimize the preparation process of antioxidant peptide with reducing power of antioxidant peptide to an index.The results showed that the most optimum preparation condition of antioxidant peptides from glutinous rice bran protein with neutral protease were substance concentration 4%, E/S 2%,pH 7,hydrolytic temperature40℃ and time 6h. The scavenging activities to ·OH and O2-· of antioxidant peptide from glutinous rice bran were stronger than which from rice bran,and their concentration and scavenging rate were definite dose-effect relationship,indicating the antioxidant peptide from glutinous rice bran could be used as functional food or natural antioxidants and be in-depth researched and developed and utilized.