Abstract: Ilex Kudingcha C.J.Tsing was a traditional tea drinks both edible and medicinal,containing a variety of active components. In order to improve the extraction efficiency of kudingcha,85% ethanol was used as solvent,and were followed by liquid-liquid extraction with equal volume of petroleum ether,ethyl acetate,chloroform and n-butanol,respectively. The petroleum ether component(PS),the acetate component(PA),chloroform component(PC),n-butanol component(PB) and the remaining portion of the water-soluble(PW)were obtained. They were identified by chemical methods and the anitoxidant activity of which were investigated both in vivo and in vitro. Results showed that the main component of ethyl acetate were phenols and flavonoids,chloroform extraction components were protein and alkaloids,n-butanol extract were protein substances,and the aqueous phase containing mainly protein and sugar,respectively. PA could significantly scavenge the DPPH free radical when the concentration was 100mg/m L,the highest removal rate was 50%followed by PW,PC and PB. In addition,PA and PW could significantly increase the activity of SOD,decreased the production of MDA in serum in vivo.