龟裂链霉菌中选择性σ因子lsigB在大肠杆菌中的异源表达及功能验证

Expression and functional analysis of an alternative sigma factor,lsig B of Streptomyces rimous in E.coli

摘要: 本文扩增了龟裂链霉菌中选择性调控因子的编码基因lsig B,并在大肠杆菌中进行异源表达。通过SDS-PAGE以及Western blotting方法鉴定Lsig B蛋白后,在多种环境压力下培养重组大肠杆菌。实验结果表明,Lsig B的表达使宿主菌对高温、渗透压、强氧化还原、乙醇以及多种抗生素胁迫的耐受性显著提高。所以Lsig B蛋白是一类能够对环境压力产生应答作用的调控因子,调控了菌体胁迫培养时的应激反应。同时本研究为深入研究Lsig B蛋白在龟裂链霉菌中的抗逆性功能奠定基础。

Abstract: In this research,the gene lsig B encoding regulatory factors from Streptomyces rimosus was heterologous expressed in E.coli.After the identification of Lsig B protein by SDS- PAGE and Western blotting,recombinant E.coli was cultured under a variety of environment stress. The results showed that the expression of Lsig B significantly enhanced the stress resistance of recombinant strains to heat,ehanol,oxygen and salt stress,together with antibiotic pressure. Therefore,the main function of Lsig B respoding to environmental stress was demonstrated.Meanwhile,the study was to lay foundation for researching the resistance function of Lsig B in Streptomyces rimosus.