Abstract: Based on static and dynamic adsorption performance, HPD-800 type macroporous resin was selected as a more appropriate carrier to separate and purify piperine from crude extract of piperine by ultrasonic.Then, recrystallize the concentrated piperine solution. The optimal process parameters for dynamic adsorption and desorption were determined as follows:30m L of 1.6mg/m L samples at natural p H and 80 m L/h of loading flow rate, followed by desorption with 50 m L 50% methanol at p H3.0. Under this condition, the purity of piperine was 82%. The purity of piperine could reach 96% at the optimal process parameters for recrystallization:piperine concentration was 200mg/m L, natural p H, 0℃ recrystallize for 24 h. Recrystallize again, the purity could up to 99%.