超高效液相色谱质谱法测定鸡肉中抗病毒类药物残留

白亚敏; 杨小珊; 毛庆; 江生; 聂旭元

doi:10.13386/j.issn1002-0306.2014.24.007

超高效液相色谱质谱法测定鸡肉中抗病毒类药物残留

白亚敏^1,2,
杨小珊^1,2,
毛庆^1,2,
江生^1,2,
聂旭元^1,2

重庆市食品药品检验所
重庆市药物过程与质量控制工程技术研究中心

基金项目:

重庆市科技攻关计划项目(cstc2012gg B10001)；

详细信息

作者简介:
白亚敏 (1977-) , 女, 硕士研究生, 工程师, 主要从事食品药品检验与研究方面的工作。;

杨小珊 (1975-) , 女, 博士研究生, 高级工程师, 主要从事食品药品检验与研究方面的工作。;

中图分类号: O657.63;TS251.55
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出版历程
- 收稿日期: 2014-03-10

Determination of antiviral drugs residue in chicken by UPLC-MS/MS

Chongqing Institute for Food and Drug Control
Chongqing Engineering Research Center for Pharmaceutical Process and Quality Control

摘要

摘要: 采用超高效液相色谱质谱（UPLC-MS/MS）法建立了鸡肉中利巴韦林和金刚烷胺等抗病毒类药物残留量的检测方法。鸡肉样品均质后,分别用乙腈-1%三氯乙酸溶液和甲醇-1%三氯乙酸溶液提取,提取液经固相萃取小柱净化,采用多反应监测模式进行检测,外标法定量。添加浓度为0.1².0μg/kg时,抗病毒类药物的回收率为66.7%⁸5.6%,相对标准偏差为3.1%⁸.5%。方法简便快捷,精密度高,重现性好,可用于鸡肉中抗病毒类药物的测定。
- 超高效液相色谱质谱法 /
- 鸡肉 /
- 利巴韦林 /
- 金刚烷胺 /
- 固相萃取
Abstract: An ultra performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) method was established to determine residue of ribavirin and amantadine in chicken. Homogenized samples were extracted separately with a mixture of acetonitrile-1% trichloroacetic acid aqueous solution and a mixture of methanol-1% trichloroacetic acid aqueous solution, and then cleaned by solid phase extraction (SPE) . UPLC-MS/MS detection was performed using multiple reaction monitoring module. The quantification was obtained by utilizing the external standard method. Recoveries of metabolites of antiviral drugs ranged from 66.7% to 85.6% at fortified levels of 0.1 ~2.0μg/kg. The RSDs of the method were in the rang of 3.1% ~8.5%. The method was simple, effective and accurate. It could be applied to determine antiviral drugs residue in chicken.
- ultra performance liquid chromatography/mass spectrometry (UPLC-MS/MS) /
- chicken /
- ribavirin /
- amantadine /
- solid phase extraction

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参考文献(11)

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