双水相萃取长鱼蛋白酶的工艺研究

Study on aqueous two- phase extraction of protease from Monopterus albus

摘要: 采用聚乙二醇(PEG)/盐双水相体系以分离纯化长鱼蛋白酶。通过研究不同成相盐及其浓度、不同PEG分子量及其浓度和不同p H对双水相萃取长鱼蛋白酶的影响。结果表明,最优化的双水相体系为25%PEG1000、20%(NH4)2SO4和p H7.0,在此条件下长鱼蛋白酶上相酶活回收率和纯化倍数分别为90.5%和4.40。

Abstract: Protease was extracted and purified by the polyethylene glycol ( PEG) - salt aqueous two- phase systems from Monopterus albus. Effects of different kinds and concentrations of phasing salts, different molecular weight and concentrations of phasing PEG, p H on extraction of the protease were studied. The results showed that the optimum conditions for extraction and purification of the protease were 25% PEG1000, 20% ( NH4) 2SO4and p H7.0.Under the optimum extraction conditions, recovery and purification fold of the protease in top phase were 90.5%and 4.40, respectively.